Field Detail

• private final java.util.List<java.lang.Integer> excitationPerChannel

  It is light that excites fluorochromes. Its the light that gets absorbed by the specimen A sufficient intensity needs to match the excitation range of the fluorochrome in order to get a signal. Measured as wavelength in nm per channel, it is stored as a list of e.g (488, 561)

Property Detail

• final java.lang.Float cameraAcquisitionTime

  Time required to generate an image with the camera Described in ms (e.g 100 ms)

• final java.util.List<EmissionRange> emissionPerChannel

  Wavelength of emission light per channel. Its the light that is emitted by the fluorophores, it describes the fluorophore signal. Measured as light as wavelength in nm per channel The emission is stored as a range of wave lengths e.g. (495-530,580-630)

  See Also:

  EmissionRange

• final java.lang.Float laserPowerBeamSplitter

  Amount of power of the laser beam split at the beam splitter. A beam splitter is an optical device that splits the beam of light It is measured in milliwatts (mW)

• final java.lang.Float laserPowerTIRFLens

  Total Internal Reflection Fluorescence (TIRF) Amount of power of the laser beam at the TIRF lens. It is measured in milliwats (mW)

• final Position position

  Position of the light beam on the scanning area Described by coordinates in pixels e.g position (x,y,z)

• final java.lang.Integer scanAcquisitionRate

  Describes the time for the acquisition of an image area in pixels. The rate is defined as time/px and has the unit ms. e.g. 5ms

• final Area scanSize

  The size of the scanned area e.g. the area (40,40) in µm of on the image plane xy

  See Also:

  Area

• final Position tcspcPosition

  Position for the TCSPC. Described by coordinates in µm e.g position (x,y,z)

• final java.lang.Integer tcspcTimingResolution

  Time-Correlated Single Photon Counting (TCSPC) A resolution is defined by the shortest distance between two points on a specimen that can still be distinguished This describe the time resolution for TCSPC Describes the timing resolution of TCSPC in ps e.g. 32 ps

• final ScanMode tscpcScanMode

  Describes the scan mode of the used for the TCSPC microscopy.

  See Also:

  ScanMode

Constructor Detail

• LightMicroscopyParameters(java.lang.Integer binning, java.util.List<java.lang.Integer> excitationPerChannel, java.util.List<EmissionRange> emissionPerChannel, java.lang.Float laserPowerTIRFLens, java.lang.Float laserPowerBeamSplitter, java.lang.Float cameraAcquisitionTime, Position position, java.lang.Integer tcspcTimingResolution, Position tcspcPosition, ScanMode tscpcScanMode, java.lang.Integer scanAcquisitionRate, Area scanSize)

  Creates a description of light microscope parameters during image analysis.

  Parameters:

  binning - LightMicroscopyParameters.binning

  excitationPerChannel - LightMicroscopyParameters.excitationPerChannel

  emissionPerChannel - LightMicroscopyParameters.emissionPerChannel

  laserPowerTIRFLens - LightMicroscopyParameters.laserPowerTIRFLens

  laserPowerBeamSplitter - LightMicroscopyParameters.laserPowerBeamSplitter

  cameraAcquisitionTime - LightMicroscopyParameters.cameraAcquisitionTime

  position - LightMicroscopyParameters.position

  tcspcTimingResolution - LightMicroscopyParameters.tcspcTimingResolution

  tcspcPosition - LightMicroscopyParameters.tcspcPosition

  tscpcScanMode - LightMicroscopyParameters.tscpcScanMode

  scanAcquisitionRate - LightMicroscopyParameters.scanAcquisitionRate

  scanSize - LightMicroscopyParameters.scanSize