Describes the properties of the super resolution microscope (SRM) hardware This class serves as DTO and contains information about general SRM hardware parameters during an image analysis. This class shall be extended, if you need to add more technology-specific properties.
| Modifiers | Name | Description |
|---|---|---|
static class |
SRMHardware.Builder |
| Modifiers | Name | Description |
|---|---|---|
private Filter |
beamSplitter |
BeamSplitters are designed to efficiently reflect excitation wavelengths and pass emission wavelengths |
private Filter |
emissionFilter |
It filters out the entire excitation range of the fluorophore under inspection |
private java.util.List<Filter> |
emissionFilterChannels |
Different channels can have different filters, this variable collects all filter applied for different channels |
private java.lang.String |
excitationBeamSplitter |
A beam splitter is an optical device that splits a beam of light in two. |
private IlluminationMode |
illuminationMode |
A microscope can have different illumination modes. |
private java.lang.Float |
laserLineFilter |
Describes the filter that has been applied to the laser lines. |
private java.lang.Integer |
laserLinesCw |
Cw stands for cool white and describes the brightness of the light / laser beam |
private java.lang.Integer |
laserLinesPulsed |
Describes the wavelength of pulsed laser lines e.g. 488 nm |
private java.lang.Integer |
laserRepetitionRate |
Describes the number of pulses per second(frequency) of a laser employed in pulse mode. |
private java.lang.String |
piezoScanner |
Scanner to detect fast, high precision motion in one or more axes |
private java.lang.String |
piezoTableController |
Piezo is greek and means 'to squeeze or press' Its the stage which 'holds' the specimen |
private Size |
pinholeSize |
This variable describes the size of the pinhole, which helps to focus the light and eliminate stray light |
private java.lang.String |
polarizationOptics |
Polarization optics change the state of polarization of incident radiation |
| Fields inherited from class | Fields |
|---|---|
class ImagingHardware |
detector, objective |
| Constructor and description |
|---|
private SRMHardware
(ProjectManager.Builder builder) |
| Methods inherited from class | Name |
|---|---|
class ImagingHardware |
getDetector, getObjective |
BeamSplitters are designed to efficiently reflect excitation wavelengths and pass emission wavelengths Beside the product name the filter used with it needs to be specified
It filters out the entire excitation range of the fluorophore under inspection Described by the controlled vocabulary of Filter
Different channels can have different filters, this variable collects all filter applied for different channels
A beam splitter is an optical device that splits a beam of light in two. Describes the name of the beam splitter hardware e.g TIRF Quad Line zt405/488/561/640rpc 514 BS LP
A microscope can have different illumination modes. It describes from where the lighting comes. The sample could be illuminated from e.g. the back or the front (direct illumination) IlluminationMode describes the applied mode with a controlled vocabulary
Describes the filter that has been applied to the laser lines. Optical filter such as e.g. Bandpass filters(BP) are employed to center the wavelength of a light source e.g 488 nm BP/LL, BP (Sem rock)
Cw stands for cool white and describes the brightness of the light / laser beam Defines the wavelength of laser lines in a continuous wave laser e.g 405 nm
Describes the wavelength of pulsed laser lines e.g. 488 nm
Describes the number of pulses per second(frequency) of a laser employed in pulse mode. Pulse frequency is measured in mega hertz (MHz), e.g 20 MHz
Scanner to detect fast, high precision motion in one or more axes The name of the scanner is defined with this parameter, e.g. P-527.3CD multi axis piezo scanner
Piezo is greek and means 'to squeeze or press' Its the stage which 'holds' the specimen The table controller enables the control of the squeezing and position of the probe plate The name of the table controller is defined with this parameter, e.g. E-710.3CD digital piezo controller
This variable describes the size of the pinhole, which helps to focus the light and eliminate stray light The Size of the pinhole is measured in µm
Polarization optics change the state of polarization of incident radiation Describes the name of the polarization optics hardware e.g Glen Thompson linear polarizer, Lambda/2 wave plate, Lambda/4 wave plate